Yes, absolutely. In fact, reading the Hanuman Chalisa at night is considered extremely powerful and beneficial. The belief that one should only pray in the morning is a misconception when it comes to Lord Hanuman.
Nighttime is traditionally associated with the rising of tamasic (negative or lethargic) energies. Chanting the Chalisa before going to sleep creates a protective energetic shield around you and your home, keeping away nightmares, fear, and negative thoughts.
After a long, stressful day, the mind is often racing with worldly anxieties. Reciting the verses calms the nervous system. The vibrations of the Chalisa act as a spiritual lullaby, shifting the mind from a state of stress to a state of surrender and devotion, leading to deep, peaceful sleep.